UNCHAINED LABS UNCLE STATIC SMOOTH DRUM ROLLER

The Unchained Labs UNCLE Static Smooth Drum Roller is an advanced protein stability analyzer designed for high-throughput, multi-parameter biophysical characterization. It supports simultaneous analysis of up to 48 samples per run with an ultra-low minimum sample volume of 0.0032 fl oz (approximately 9 μL) per sealed capillary. The system covers a broad sample concentration range of 0.05–300 mg/mL, enabling versatile application across diverse protein types. Temperature control is precise, ranging from 59 to 203 °F (15–95 °C), with an accuracy of ±0.18 °F and a programmable heating rate from 0.18 to 18 °F/min, supporting detailed thermal stability profiling. UNCLE integrates three optical measurement modes: full-spectrum fluorescence, static light scattering (SLS), and dynamic light scattering (DLS). It excites samples at dual wavelengths of 266 nm and 473 nm with fluorescence detection across a broad spectrum from 250 to 720 nm. DLS capability delivers hydrodynamic diameter measurements from 0.000012 to 0.03937 inches (0.3–1000 nm) with a size accuracy of ±0.000079 inches, along with molecular weight determination ranging from 0.000424 to 0.055115 lb (~192 Da to 25 MDa). The minimum sample concentration required for DLS sizing is 0.1 mg/mL. This instrument offers comprehensive protein characterization metrics, including thermal melting temperature (Tm), aggregation temperature (Tagg), sizing, polydispersity, viscosity, molecular interaction parameters (kD, B22, G22), and refolding analysis. Its compact design (approx. 54 cm x 50 cm x 58 cm, 50 kg) combines high sensitivity and throughput, significantly reducing sample consumption and experiment time in protein stability and formulation studies. The UNCLE roller thus serves as a powerful tool for streamlined, multi-modal protein biophysical analysis in research and development settings.